Part 2: So the greasy spot of the pipette from jar A is bigger than the
spot of the pipette from jar B, although jar B contains more oil. I think
you couldn't control the suck by the pipette, it's every time a different
proportion of oil and water. I tried this experiment several times with
different quantities of oil and the results, taken by the greasy spot test,
weren't comparable with the amount of oil in the jars. Or do you take
liquid with the pipette under the oil film?
Roman Remember oil will float on water - at least in the beginning. My
methodology: First I made sure I took the sample from the same part of the
beaker each time. I placed the pipette just below the surface - about 1mm
and took a small sample - ie oil floats on water. I placed 4 drops from the
pipette onto brown paper and waited for it to dry. Any oil in the liquid
left a stain on the paper If there was no oil in the sample there will be
no stain More in next reply Jason
Part 4: Could it be that this foam is the dispersion-zone where the
bacterias "attack" the little oil drops? At the end of the experiment
(after 4 weeks) I took the foam of each jar out and put it in a little
glazier (picture S5A). After I centrifuged it, there was a white stable,
thick shift (picture Ö5A), which looked like protein. Do you think that
this shift contains the bacterias, because bacterias are build by protein,
and when you shake protein it get fixed?
I am a high school student from Switzerland have done my thesis on oil
degradation by bacterias. I tried in my experiment the greasy spot test for
meassuring the oil level in each jar, but it didn't worke. So I took
another methode. But I'm still wondering how the greasy spot test works and
I have no idea how you can be sure that you take the same proportion of oil
and water in each jar. It would be nice if you can give me an answer.
To make it more clear, the jars have an equal amount of oil in them at the
start, the grease spot test can determine if the oil is decreasing by means
of the pipette sucking up more water per oil. Since the oil will be
decreasing, less will be available at the top of the water to take from,
making the water more likely to be sucked up. Leaving smaller grease spots.
It's not a perfect test, but it's still an interesting method.
Thanks for your reply. But for me it's still not clear how this method
could work. An example: you have two jars, in jar A is 1.5ml oil and in jar
B is 2ml oil, both contains the same quantity of water. If you want to show
the difference between the two oil quantities with the greasy spot test, it
doesn't work. Perhaps the pipette takes in jar A more oil than in jar B and
in jar B more water than in jar A.
Part 3: Another question about the experiment you have done in the video
and I have done for my thesis. During the experiment it builds up foam by
reason of the aquarium pumps and this foam is under the oil level. What do
you think are the ingredients of this foam? (Look at picture 2C 12.6)
Part 2 After I while I suspect some of the oil forms clumps and sinks to
the bottom or it adsorbs onto the soil particles. These were questions I
posed in the second video for this experiment and which you could test for.
Jason
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